ntc buffer (MACHEREY NAGEL)
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MACHEREY NAGEL
ntc buffer
Ntc Buffer, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 95/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntc+buffer/NucleoSpin+Plasmid+Buffer+Set/us12606814-3755-11-14
Average 95 stars, based on 189 article reviews
Ntc Buffer, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 95/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntc+buffer/NucleoSpin+Plasmid+Buffer+Set/us12606814-3755-11-14
Average 95 stars, based on 189 article reviews
ntc buffer - by Bioz Stars,
2026-10
95/100 stars
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Purification:Article Title: High-Fidelity and Cost-Effective Engineering of SARS-CoV-2 Article Snippet: Total RNA was extracted with Trizol (Invitrogen, Carlsbad, CA, USA) using the manufacturer′s recommendations and the totality of the viral RNA was treated with Turbo DNase (Thermo Fisher Scientific, Waltham, MA, USA) for 30 min at 37 ◦C. .. Following DNase treatment, RNA was column purified using Article Title: [2512.20581] MERGE-RNA: a physics-based model to predict RNA secondary structure ensembles with chemical probing Article Snippet: PCR was performed in 50 μL reactions containing the following final concentrations: GXL reaction buffer (1×, from a 5× stock; Takara Bio), each dNTP (0.2 mM), each primer (0.25 μM; FullT7-RV and Full-FW), single-stranded full-length DNA template (1 nM), and PrimeSTAR GXL DNA Polymerase (0.025 U/μL; Takara Bio). .. Thermal cycling conditions were as follows: initial denaturation at 98 ◦C for 2 min; 25 cycles of 98 ◦C for 20 s, 55 ◦C for 20 s, and 68 ◦C for 30 s; followed by a final extension at 68 ◦C for 5 min. PCR products were analyzed on a 2% agarose gel to verify the expected size, before purification using the NucleoSpin Gel and PCR Clean-up kit with Article Title: Evolutionary adaptation of bacterial proteomes to translation-impeding sequences Article Snippet: The translation reaction mixture was then mixed with the same volume of the reverse transcription mixture containing 50 mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13 mM magnesium acetate, 1 mM DTT, 2 μM of oligonucleotide labeled with 6-carboxyfluorescein (6-FAM) at the 5’ end (5’-AACGACGGCCAGTGAATCCGTAATCATGGT-3’, Invitrogen), 50 μM each dNTP, and 10 U/μL ReverTra Ace (Toyobo), then incubated further at 37 °C for 15 min. .. The reaction mixture was diluted fivefold with the Article Title: Evolutionary adaptation of bacterial proteomes to translation-impeding sequences. Article Snippet: The translation reaction mixture was then mixed with the same volume of the reverse transcription mixture containing 50mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13mMmagnesium acetate, 1 mM DTT, 2 μM of oligonucleotide labeled with 6-carboxyfluorescein (6-FAM) at the 5’ end (5’- AACGACGGCCAGTGAATCCGTAATCATGGT-3’, Invitrogen), 50 μM each dNTP, and 10U/μL ReverTra Ace (Toyobo), then incubated further at 37 °C for 15min. .. The reaction mixture was diluted fivefold with the Article Title: A mini-hairpin shaped nascent peptide blocks translation termination by a distinct mechanism Article Snippet: The in vitro translation reaction sample was mixed with an equal volume of reverse transcription mixture [50 mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13 mM magnesium acetate, 1 mM DTT, 2 μM fluorescently labeled oligonucleotide (pe-lacZ-N-rv with Alexa 647 at 5’-terminus), 40 μM of each dNTP, 10 unit/μl ReverTra Ace (Toyobo)] and incubated at 37 °C for 10 min. .. The reverse transcription products were purified by a NucleoSpin Gel and PCR clean-up kit equilibrated with Polymerase Chain Reaction:Article Title: High-Fidelity and Cost-Effective Engineering of SARS-CoV-2 Article Snippet: Total RNA was extracted with Trizol (Invitrogen, Carlsbad, CA, USA) using the manufacturer′s recommendations and the totality of the viral RNA was treated with Turbo DNase (Thermo Fisher Scientific, Waltham, MA, USA) for 30 min at 37 ◦C. .. Following DNase treatment, RNA was column purified using Article Title: [2512.20581] MERGE-RNA: a physics-based model to predict RNA secondary structure ensembles with chemical probing Article Snippet: PCR was performed in 50 μL reactions containing the following final concentrations: GXL reaction buffer (1×, from a 5× stock; Takara Bio), each dNTP (0.2 mM), each primer (0.25 μM; FullT7-RV and Full-FW), single-stranded full-length DNA template (1 nM), and PrimeSTAR GXL DNA Polymerase (0.025 U/μL; Takara Bio). .. Thermal cycling conditions were as follows: initial denaturation at 98 ◦C for 2 min; 25 cycles of 98 ◦C for 20 s, 55 ◦C for 20 s, and 68 ◦C for 30 s; followed by a final extension at 68 ◦C for 5 min. PCR products were analyzed on a 2% agarose gel to verify the expected size, before purification using the NucleoSpin Gel and PCR Clean-up kit with Article Title: Evolutionary adaptation of bacterial proteomes to translation-impeding sequences Article Snippet: The translation reaction mixture was then mixed with the same volume of the reverse transcription mixture containing 50 mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13 mM magnesium acetate, 1 mM DTT, 2 μM of oligonucleotide labeled with 6-carboxyfluorescein (6-FAM) at the 5’ end (5’-AACGACGGCCAGTGAATCCGTAATCATGGT-3’, Invitrogen), 50 μM each dNTP, and 10 U/μL ReverTra Ace (Toyobo), then incubated further at 37 °C for 15 min. .. The reaction mixture was diluted fivefold with the Article Title: Evolutionary adaptation of bacterial proteomes to translation-impeding sequences. Article Snippet: The translation reaction mixture was then mixed with the same volume of the reverse transcription mixture containing 50mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13mMmagnesium acetate, 1 mM DTT, 2 μM of oligonucleotide labeled with 6-carboxyfluorescein (6-FAM) at the 5’ end (5’- AACGACGGCCAGTGAATCCGTAATCATGGT-3’, Invitrogen), 50 μM each dNTP, and 10U/μL ReverTra Ace (Toyobo), then incubated further at 37 °C for 15min. .. The reaction mixture was diluted fivefold with the Article Title: A mini-hairpin shaped nascent peptide blocks translation termination by a distinct mechanism Article Snippet: The in vitro translation reaction sample was mixed with an equal volume of reverse transcription mixture [50 mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13 mM magnesium acetate, 1 mM DTT, 2 μM fluorescently labeled oligonucleotide (pe-lacZ-N-rv with Alexa 647 at 5’-terminus), 40 μM of each dNTP, 10 unit/μl ReverTra Ace (Toyobo)] and incubated at 37 °C for 10 min. .. The reverse transcription products were purified by a NucleoSpin Gel and PCR clean-up kit equilibrated with other:Article Title: Evolutionary adaptation of bacterial proteomes to translation-impeding sequences Article Snippet: Reverse Transcription:Article Title: A mini-hairpin shaped nascent peptide blocks translation termination by a distinct mechanism. Article Snippet: The in vitro translation reaction samplewasmixedwith an equal volumeof reverse transcription mixture [50mM HEPES-KOH, pH 7.6, 100mM potassium glutamate, 2mM spermidine, 13mM magnesium acetate, 1mM DTT, 2 μM fluorescently labeled oligonucleotide (pe-lacZ-N-rv withAlexa 647 at 5’-terminus), 40 μMof eachdNTP, 10 unit/μl ReverTra Ace (Toyobo)] and incubated at 37 °C for 10min. .. The reverse transcription productswere purifiedby aNucleoSpinGel and PCRclean-up kit equilibrated with Article Title: Evolutionary adaptation of bacterial proteomes to translation-impeding sequences Article Snippet: The translation reaction mixture was then mixed with the same volume of the reverse transcription mixture containing 50 mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13 mM magnesium acetate, 1 mM DTT, 2 μM of oligonucleotide labeled with 6-carboxyfluorescein (6-FAM) at the 5’ end (5’-AACGACGGCCAGTGAATCCGTAATCATGGT-3’, Invitrogen), 50 μM each dNTP, and 10 U/μL ReverTra Ace (Toyobo), then incubated further at 37 °C for 15 min. .. The reaction mixture was diluted fivefold with the Article Title: Evolutionary adaptation of bacterial proteomes to translation-impeding sequences. Article Snippet: The translation reaction mixture was then mixed with the same volume of the reverse transcription mixture containing 50mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13mMmagnesium acetate, 1 mM DTT, 2 μM of oligonucleotide labeled with 6-carboxyfluorescein (6-FAM) at the 5’ end (5’- AACGACGGCCAGTGAATCCGTAATCATGGT-3’, Invitrogen), 50 μM each dNTP, and 10U/μL ReverTra Ace (Toyobo), then incubated further at 37 °C for 15min. .. The reaction mixture was diluted fivefold with the Article Title: A mini-hairpin shaped nascent peptide blocks translation termination by a distinct mechanism Article Snippet: The in vitro translation reaction sample was mixed with an equal volume of reverse transcription mixture [50 mM HEPES-KOH, pH 7.6, 100 mM potassium glutamate, 2 mM spermidine, 13 mM magnesium acetate, 1 mM DTT, 2 μM fluorescently labeled oligonucleotide (pe-lacZ-N-rv with Alexa 647 at 5’-terminus), 40 μM of each dNTP, 10 unit/μl ReverTra Ace (Toyobo)] and incubated at 37 °C for 10 min. .. The reverse transcription products were purified by a NucleoSpin Gel and PCR clean-up kit equilibrated with Agarose Gel Electrophoresis:Article Title: [2512.20581] MERGE-RNA: a physics-based model to predict RNA secondary structure ensembles with chemical probing Article Snippet: PCR was performed in 50 μL reactions containing the following final concentrations: GXL reaction buffer (1×, from a 5× stock; Takara Bio), each dNTP (0.2 mM), each primer (0.25 μM; FullT7-RV and Full-FW), single-stranded full-length DNA template (1 nM), and PrimeSTAR GXL DNA Polymerase (0.025 U/μL; Takara Bio). .. Thermal cycling conditions were as follows: initial denaturation at 98 ◦C for 2 min; 25 cycles of 98 ◦C for 20 s, 55 ◦C for 20 s, and 68 ◦C for 30 s; followed by a final extension at 68 ◦C for 5 min. PCR products were analyzed on a 2% agarose gel to verify the expected size, before purification using the NucleoSpin Gel and PCR Clean-up kit with |